Other
Part:BBa_K940000
Designed by: Louise Usher Group: iGEM12_Westminster (2012-09-25)
ALDH1A1 promoter in a pSB1C3 backbone
This part is a promoter for ALDH1A1,an enzyme involved in alcohol metabolism. Nucleotide sequences were analysed on NCBI. Sequences were selected by determining the start of the protein coding region and then taking the previous 1000 bases which included the TAATAA box. The sequences selected were compared with commercial sequences. Additionally overlap between the selected sequence and sequences on the Eukaryotic Promoter database (EPD) was confirmed. ALDH sequence was selected to exclude BioBrick restriction sites.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |